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StayBrite™ Highly Stable ATP Bioluminescence Assay Kit

based on 1 citations in multiple journalsStayBrite™ Highly Stable ATP Bioluminescence Assay Kit14.1 4
Highly Sensitive, Stable Assay, HTS
Catalog #: K791
SKU-Size Size Price Qty
K791-100 100 assays
K791-1000 1000 assays
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Product Details

Detection Method Luminescence
Species Reactivity Mammalian
Applications The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).
Features & Benefits • Stable for >10 hours at room temperature and >60 minutes at 37°C.
• The stable reagent provides more accuracy than any other ATP assay kits.
Kit Components • StayBrite™ Reaction Buffer
• StayBrite™ Reconstitution Buffer
• StayBrite™ Enzyme Mix (lyophilized)
• ATP Standard (MW 551) (lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Adenosine-5’-triphosphate (ATP) plays an important role in living systems. It has also been used as an indicator to monitor many biological processes and disease stages, etc.. An accurate and reliable detection of ATP level has wide applications. Luciferase/Luciferin has been used to sensitively measure ATP levels. Conventional Luciferase/Luciferin ATP detection systems, however, are unstable. Luciferase loses activity rapidly. At BioVision, we have developed a highly stable Luciferase, which is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. We named the highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, the rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH’s below ~8.2, rLucHS is significantly more active than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5x10ᶦᶦRLU/mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.

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Can I use BCA assay to measure the sample protein concentration. Samples are made in 2X Reaction Buffer.
The 2X Reaction Buffer contains BSA. BSA is used as a Standard in both BCA as well as Bradford assay. For measuring the protein concentration using any of these methods, we recommend using the 2X Reaction Buffer as a blank, which then needs to be subtracted from the sample OD. The protein concentration can also be measured using Qubit or Nanodrop.