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SSA IgG (Human) ELISA Kit

An ELISA kit for quantitative measurement of SSA IgG in human serum and plasma samples.

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

Catalog #: E4979
$695.00

Product Details

Cat # +Size E4979-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Features & Benefits ● Easy, convenient and time-saving method to measure the amount of SSA IgG in serum and plasma samples
● No significant cross-reactivity or interference with other autoantibodies was observed
Kit Components ● Micro ELISA plate coated with SSA antigen
● Sample Diluent
● Calibrator
● Positive Control
● Negative Control
● Enzyme conjugate
● TMB Substrate
● Wash Buffer (20X)
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sjogren syndrome (SS)A/Ro are autoantigens that are a type of extractable nuclear antigen (ENA). SSA consists of 2 proteins: Ro60 and Ro52. Ro60 binds to uncoded RNA to form an hY-RNA complex and thereby inhibits the immune response. Ro52 is a protein formed either due to viral infection, type I interferons, or Toll-like receptors. Antibodies to SSA/Ro antigen are detected in patients suffering from autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren's syndrome (SS), systemic sclerosis, and rheumatoid arthritis (RA). BioVision’s SSA IgG (Human) ELISA kit quantitatively measures SSA IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with SSA antigen. IgG antibodies to SSA, if present in the samples, will bind to the SSA antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to SSA present in the sample


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