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Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)

Highly Sensitive Assay for the detection of Soluble Epoxide Hydrolase; Includes sEH Positive Control
Catalog #: K477

In stock

$455.00

Product Details

Cat # +Size K477-100
Size 100 assays
Kit Summary • Detection method-Fluorescence (Ex/Em: 362/460 nm)
• Applications- Measurement of sEH activity in cell and tissue lysates using a 96-well plate format
Detection Method Fluorescence (Ex/Em: 362/460 nm)
Species Reactivity Eukaryotes
Applications • Rapid assessment of native/recombinant sEH activity.
•  Soluble Epoxide Hydrolase activity in mammalian cell culture, and tissue lysates.
Features & Benefits • Rapid, simple & sensitive
• Convenient & HTS
• Includes specific sEH inhibitor
Kit Components • sEH Assay Buffer
• sEH Substrate
• sEH Inhibitor (5X)
• Fluorescence Standard
• sEH Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Soluble Epoxide Hydrolase or sEH (EC 3.3.2.10) is a cytosolic enzyme present ubiquitously in several organs including the liver, kidney, pancreatic islets, pituitary gland, lymphoid tissues, muscles, and the gastrointestinal tract. It catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), i.e., epoxides derived from cytochrome P-450 mediated metabolism of arachidonic acid, and forming vicinal diols. sEH plays a major role in metabolism of endogenous lipids that are implicated in pain and inflammation. BioVision’s Soluble Epoxide Hydrolase Activity Assay Kit is a microplate based fluorometric kit for measuring sEH activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activitys.


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