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Sodium Assay Kit (Colorimetric)

The most sensitive kit on the market
Catalog #: K391
$615.00

Product Details

Cat # +Size K391-100
Size 100 assays
Detection Method Absorbance (405 nm)
Applications The kit can detect Sodium concentration as low as 25 µM in a variety of samples.
Features & Benefits • Simple procedure; takes ~ 3 hours
• Fast and convenient
• The assay is sensitive and stable
• The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit Components • Na Assay Buffer
• Substrate
• βG
• DTT (1 M)
• Na Developer
• Na Standard (1.5 M)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sodium (Na) is one of the most important electrolytes along with chloride, calcium and potassium. Na plays vital roles in the maintenance of plasma volume, pH balance, transmission of nerve impulses, and normal cell functions. Healthy individuals can absorb sodium ingested in food, and kidneys maintain proper sodium balance by excreting its excess in urine. Sodium sources include table salt, milk, meat, shellfish, bread, snack food, etc. Normal Sodium intake has been defined to be between 200-500 mg/day. Patients suffering high blood pressure, hypertension, chronic kidney disease, and people suffering salt sensitivity require restricted low-sodium diets due to those conditions. Hyponatremia (low sodium concentration in blood) can occur in patients with nephrotic syndrome, excessive vomiting and diarrhea, while Hypernatremia (high sodium concentration in blood) is developed in patients suffering from liver diseases, burns, and pregnancy. Traditionally, sodium concentration in clinical settings is determined by potentiometric, gravimetry, photometry, titrimetry and flame atomic emission spectroscopy, but these methods require expensive and complex protocols that need to be performed by trained personnel. BioVision’s Sodium Assay Kit offers a simple, two-step colorimetric assay that is based on the requirement of sodium ion as a cofactor for the enzymatic activity of β-Galactosidase (βG). Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay. The kit can detect Sodium concentration as low as 25 µM in a variety of samples.


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How to prepare a sample background control?
Dilute your sample 1:100 or 1:200 in Assay buffer+DTT. Add 40 µl of substrate, mix well and incubate at 37C for 30min protected from light. After incubation add 100ul of Na Developer and mix well. Measure absorbance at OD405nm.
What is the volume (and dilution factor) needed to use urine in this kit?
For urine we recommend 25-200x dilution and then testing 1-40 ul volume sample per well.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.