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Sm/RNP IgG (Human) ELISA Kit

An ELISA kit for quantitative measurement of Sm/RNP IgG in human serum and plasma samples.

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4981
$695.00

Product Details

Cat # +Size E4981-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Features & Benefits ● No significant cross-reactivity or interference with other autoantibodies was observed
● Easy, convenient and time-saving method to measure the amount of Sm/RNP IgG in serum and plasma samples
Kit Components ● Micro ELISA plate coated with Sm/RNP antigen
● Sample Diluent
● Calibrator
● Positive Control
● Negative Control
● Enzyme conjugate
● TMB Substrate
● Wash Buffer (20X)
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Smith/Ribonucleoprotein (Sm/RNP, U1RNP) are autoantigens that are a type of extractable nuclear antigen (ENA). Antibodies to Sm/RNP target small nuclear ribonucleoproteins (snRNP). snRNPs are localized in the nucleus and play an important role in the processing of pre-mRNA. Antibodies to RNP target 3 autoantigens (A, C, and 68 kD); whereas antibodies to Sm target 7 autoantigens (B/B', D1, D2, D3, E, F, G). Antibodies to Sm are detected in 20% of the patients suffering from systemic lupus erythematosus (SLE). On the other hand, antibodies to RNP are detected in 45% of the patients with SLE and also in patients suffering from Sjögren’s syndrome, scleroderma, mixed connective tissue disease, and polymyositis. BioVision’s Sm/RNP IgG (Human) ELISA kit quantitatively measures Sm/RNP IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with Sm/RNP antigen. IgG antibodies to Sm/RNP, if present in the samples, will bind to the Sm/RNP antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to Sm/RNP present in the sample.


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