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SLC40A1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of SLC40A1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4988
$795.00

Product Details

Cat # +Size E4988-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure SLC40A1 in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● Sensitivity: 0.094 ng/ml
● Detection range: 0.156 – 10 ng/ml
● This Sandwich ELISA is highly sensitive and highly specific for the detection of SLC40A1 in human samples. There is no significant cross-reactivity or interference between SLC40A1 and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Ferroportin-1 (FPN1, IREG1, SLC40A1) is a transmembrane protein that belongs to the ferroportin family of proteins. It is the only known iron exporter in mammals. After absorption of dietary iron in the small intestine, SLC40A1 exports the iron out of the intestinal cells into the bloodstream. It is expressed in cells such as macrophages, hepatocytes, duodenal enterocytes, placental syncytiotrophoblasts, and adipocytes. However, it is absent in erythroid cells. Expression of SLC40A1 is regulated by Hepcidin, a liver-derived hormone that causes internalization and degradation of SLC40A1 and reduces the efflux of iron into the bloodstream. Both Hepcidin and SLC40A1 play a critical role in the pathogenesis of iron metabolism diseases, iron overload, and iron-limited anemia. Mutations in SLC40A1 result in an autosomal dominant disorder called type IV hemochromatosis or Ferroportin Disease. BioVision’s SLC40A1 (Human) ELISA kit quantitatively measures SLC40A1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of SLC40A1 in the sample or standard.


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