Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

SIRT3 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of SIRT3 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5009
$795.00

Product Details

Cat # +Size E5009-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of SIRT3 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Recovery range: 85 - 105% for normal human serum and plasma samples
● This Sandwich ELISA is highly sensitive and highly specific for the detection of SIRT3 in human samples. There is no significant cross-reactivity or interference between SIRT3 and analogues
● Detection range: 0.156 – 10 ng/ml
● Sensitivity: 0.094 ng/ml
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sirtuin3 (SIRT3) is one of the seven homologs of the yeast histone deacetylase, silent information regulator (Sir2). This NAD+-dependent deacetylase is predominantly localized in the mitochondria, and its major function to maintain homeostasis in mitochondria under stress conditions. SIRT3 is highly expressed in tissues such as the liver, kidney, skeletal muscle, cardiac muscle, and brown adipose tissue. The activity of SIRT3 is regulated by the availability of nutrients. Under fasting conditions, SIRT3 activates TCA cycle enzymes and promotes the switch from anabolic (glycolysis) to catabolic metabolism (β oxidation of fatty acids). SIRT3 also regulates inflammation, apoptosis, autophagy, and oxidative stress. Several studies have demonstrated a critical role of SIRT3 in several diseases such as Type 2 diabetes, aging, liver, kidney, inflammatory, cardiovascular, and neurodegenerative diseases. SIRT3 also plays a dual role in cancer; however, the role is tumor-specific. Due to its critical role in several human diseases, SIRT3 could serve as an efficient therapeutic target. Several small-molecule compounds are under investigation that target SIRT3 to treat human diseases. BioVision’s SIRT3 (Human) ELISA kit quantitatively measures SIRT3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of SIRT3 in the sample or standard.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.