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SIRT1 Inhibitor/Activator Screening Kit (Fluorometric)

Simple assay for high-throughput screening of SIRT1 inhibitors/activators
Catalog #: K325
$550.00

Product Details

Cat # +Size K325-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 400/505 nm)
Applications Screening/characterizing/studying SIRT1 inhibitors/activators
Features & Benefits • Simple and reliable test to screen SIRT1 inhibitors/activators
• High-throughput suitable
• Includes Inhibitor Control (Nicotinamide)
Kit Components • SIRT1 Assay Buffer
• 1 M DTT
• Substrate (in DMSO)
• NAD
• SIRT1 Enzyme
• Inhibitor (Nicotinamide, 4 mM)
• Developer
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

SIRT1 or Sirtuin 1 is a member of the silent information regulator 2 family. SIRT1, an NAD-dependent histone deacetylase can deacetylate histones and a number of nonhistone substrates, including p53. It is predominantly localized in the nucleus of normal cells. In cancer cells however, it is predominantly localized in the cytoplasm. SIRT1 has been shown to regulate a wide range of cellular functions that affect metabolic homeostasis and aging. SIRT1 exerts anti-apoptotic, anti-oxidative, and anti-inflammatory effects against cellular injury, and protects the cells through the regulation of mitochondrial biogenesis, autophagy, and metabolism in response to the cellular energy and redox status. SIRT1 also promotes vasodilation and protects vascular tissues. Activation and inhibition of SIRT1 is being targeted for various diseases. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. In BioVision’s SIRT1 Inhibitor/Activator screening Kit, Sirtuin 1 deacetylates the substrate, followed by cleavage of the deacetylated substrate to release the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. In the presence of SIRT1 inhibitor, deacetylation is impeded, preventing cleavage of the substrate and release of the fluorescent group. The SIRT1 activator enhances SIRT1 activity resulting in a higher fluorescent signal in comparison to the control. This kit provides a rapid, simple, sensitive, and reliable test, which is suitable for high-throughput screening of SIRT1 inhibitors/activators. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.