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SIRPA / CD172a (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of SIRPA in human serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4554

In stock

$695.00

Product Details

Cat # +Size E4554-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of SIRPA in human serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of SIRPA in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of SIRPA in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.156 - 10 ng/ml
• Sensitivity: < 0.094 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

SIRPA is an immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. BioVision’s SIRPA ELISA kit is a sandwich ELISA assay for the quantitative measurement of SIRPA in human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of SIRPA captured from the samples.


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