Product Details
| abID | ab50092 |
|---|---|
| Alternate Name | soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen |
| Gene Symbol | FASL |
| Gene ID | 355 |
| Accession # | P25445 |
| Source | E. coli. |
| Appearance | Lyophilized powder |
| Physical Form Description | Sterile filtered through a 0.2 micron filter. Lyophilized with no additives. |
| Molecular Weight | 17.6 kDa |
| Purity by SDS-PAGE | ≥98% |
| Endotoxin Level | < 0.1 ng/μg of protein (<1EU/μg). |
| Biological Activity | ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 μg/ml in the presence of 2ng/ml of hFasL. |
| Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
| Amino Acid Sequence | MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR homologous cysteine rich extracellular domain Fas.
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