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SETDB1 Antibody

Polyclonal antibody to detect SETDB1 in human and mouse samples.
Catalog #: 6155
$335.00

Product Details

Cat # +Size 6155-100
Size 100 μg
Antibody Target SETDB1
Alternate Name ESET, H3­K9­HMTase4, KG1T, KIAA0067, KMT1E.
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Fusion protein of human SETDB1
Accession # Q15047
Gene ID 9869
Appearance Colorless liquid
Concentration 1 mg/ml
Formulation 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification Affinity purified
Species Reactivity Human, mouse
Application Western blot, Immunohistochemistry, immunoprecipitation
Positive Control Western Blot: Various cell lysates
Application & Usage Western blot: 1:500 – 1:1000, IHC: 1:50 – 1:100, IP: 1:50 – 1:100
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The Erg­associated protein with SET domain (ESET), also known as SET domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila, Enhancer of zeste, and Trithorax proteins. ESET also contains tudor and methyl­CpG­binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing. However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters. ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co­repressors (mSin3A/B, MBD1, KAP­1, the ATFa­associated modulator mAM). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di­methyl to tri­methyl histone H3 Lys9. MBD1 recruits ESET to the CAF­1 complex to facilitate methylation of histone H3 Lys9 during replication­coupled chromatin assembly in S phase. DNMT3A recruits ESET to silenced promoters in cancer cells. ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri­methyl histone H3 Lys9 are both increased in diseased brains.


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