Serum Amyloid A (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Serum Amyloid A (SAA) in human plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.p65warnings.ca.gov/.
Catalog #: E4937 | abID:

Product Details

Cat # +Size E4937-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of SAA in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • This Sandwich ELISA is highly sensitive and highly specific for the detection of SAA in human samples. There is no significant cross-reactivity or interference between SAA and analogues
• Recovery range: 85 - 105% for normal human serum and plasma samples
• Sensitivity: 0.938 ng/ml
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Detection range: 1.563 - 100 ng/ml
Kit Components • Micro ELISA plate
• Wash Buffer (25X)
• Plate Sealers
• Standard (Lyophilized) (100 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Serum Amyloid A (SAA) is an acute-phase plasma protein that belongs to the family of apolipoproteins. Pro-inflammatory cytokines such as IL-6, TNF, TGF-β, and interferon-γ stimulate the production of SAA. The concentration of SAA increases 1000 fold within 5-6 hours of inflammation or injury. During the acute phase of inflammation, it forms a complex with plasma high-density lipoproteins, reroutes HDL transport to promote clearance of lipids from the injury site, and recycle lipids for tissue repair. The liver is the primary source of circulating SAA; however, SAA may also be produced by extrahepatic tissues. High levels of SAA have been reported in pathological diseases such as cancer, diabetes, obesity, Alzheimer’s disease, atherosclerosis, and rheumatoid arthritis. Increased levels of SAA also help predict the progression of COVID-19 disease. Therefore, SAA serves as an efficient biomarker for the treatment of human diseases. BioVision’s Serum Amyloid A (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of SAA in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of SAA in the sample or standard


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