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Senescence Detection Kit (Fluorometric)

Simple, sensitive, one-step assay to measure senescence cells using FACS
Catalog #: K991
$615.00

Product Details

Cat # +Size K991-100
Size 100 assays
Detection Method FACS (FL1 channel)
Species Reactivity Mammalian
Applications Detection of senescence cells
Features & Benefits Simple, one step protocol
Kit Components • Wash Buffer
• Senescence Dye
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of β-galactosidase (SA-β-Gal) activity, and altered patterns of gene expression. The Senescence detection kit is designed to fluorescently detect SA-β-Gal activity in cultured cells by FACS. The SA-β-Gal is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells


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What is the principle of the assay and why is it that I see staining in my normal cells also?
The assay is based on the hydrolysis of a membrane permeable dye by beta-galactosidase, which is enriched in senescent cells. After hydrolysis and laser excitation, the dye emits fluorescence which can be detected by flow cytometry. This assay is not a black and white assay. You will see some background staining in normal, control or untreated cells as compared to senescent cells. But the staining is strong and more in senescent cells as compared to the pre-senescent, quiescent or immortal cells. To make the detection easier, once can use different control cells such as cells either treated with senescent inducing agents or late passage cells, which are believed to be more senescent as compared to early passage cells.