Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

New

SCL-70 IgG (Human) ELISA Kit

An ELISA kit for quantitative measurement of SCL-70 IgG in human serum and plasma samples.

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4980
$695.00

Product Details

Cat # +Size E4980-100
Size 96 assays
Species Reactivity Human
Features & Benefits ● Easy, convenient and time-saving method to measure the amount of SCL-70 IgG in serum and plasma samples
● No significant cross-reactivity or interference with other autoantibodies was observed
Kit Components ● Micro ELISA plate coated with SCL-70 antigen
● Sample Diluent
● Calibrator
● Positive Control
● Negative Control
● Enzyme conjugate
● TMB Substrate
● Wash Buffer (20X)
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

DNA Topoisomerase I is an important nuclear enzyme that unwinds the double-stranded DNA, relaxes, and reanneals the strand. Initially, the enzyme is synthesized as a 100 kDa protein. However, this precursor undergoes proteolytic cleavage to yield a 70 kDa form, which is also known as SCL-70. Antibodies to SCL-70 antigen are detected in 20% of the patients suffering from diffused scleroderma, and in 10% of the patients suffering from systemic scleroderma (CREST Syndrome). The presence of SCL-70 antibodies has also been reported in patients suffering from systemic lupus erythematosus (SLE). BioVision’s SCL-70 IgG (Human) ELISA kit quantitatively measures SCL-70 IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with SCL-70 antigen. IgG antibodies to SCL-70, if present in the samples, will bind to the SCL-70 antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to SCL-70 present in the sample.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.