SARS-CoV-2 RBD ELISA Kit (ab289833)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Nasal Lavage Supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
SARS-CoV-2 RBD ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 12% Inter-assay Sample n Mean SD CV% Overall < 15% -
Sample type
Nasal Lavage Supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
12.5 pg/ml - 800 pg/ml -
Assay duration
Multiple steps standard assay -
Product overview
This SARS-CoV-2 RBD ELISA Kit (ab289833, E4877) is designed to quantitatively measure the amount of RBD protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples. The assay is based on the Sandwich ELISA principle. Test samples, Standards, and Detection A solution are added to the wells pre-coated with the RBD antibody and then washed with Wash Buffer. The Detection B solution is added and any unattached conjugates are washed off using Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of RBD in the sample or standard.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 96 tests Detection A 1 vial 1 vial Detection B 1 vial 1 vial Microtiter ELISA plate 1 unit 1 unit Plate Sealer 4 units 4 units Reagent Dilution Buffer 1 x 25ml 1 x 25ml SARS-CoV-2 RBD Standard (40 ng) 1 vial 1 vial Standard/Sample Dilution Buffer 1 x 25ml 1 x 25ml Stop Solution 1 x 6ml 1 x 6ml TMB substrate (Avoid light) 1 x 12ml 1 x 12ml Wash Buffer (20X) 1 x 25ml 1 x 25ml -
Research areas
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289833 has not yet been referenced specifically in any publications.