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Product Details
| Cat # +Size | E4877-100 |
|---|---|
| Size | 96 assays |
| Detection Method | Absorbance (450 nm) |
| Applications | Sandwich ELISA kit to Quantitatively determine the concentration of RBD protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples |
| Features & Benefits | • Sensitivity: < 10 pg/ml
• Detection range: 12.5 – 800 pg/ml • This Sandwich ELISA is highly sensitive and highly specific for the detection of RBD protein in bronchoalveolar lavage fluid and nasopharyngeal samples • Assay Precision: Intra-Assay CV < 12% and Inter-Assay CV < 15% |
| Kit Components | • Microtiter ELISA plate
• SARS-CoV-2 RBD Standard (1600 pg) • Standard/Sample Dilution Buffer • RBD Biotin Conjugated Antibody • Reagent Dilution Buffer • HRP-Streptavidin Conjugate (SABC) • TMB Substrate Solution • Stop Solution • Wash Buffer (20X) • Plate Sealer |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
COVID-19 disease is a respiratory illness caused by a novel coronavirus called systemic acute respiratory syndrome coronavirus-2 (SARS-CoV-2). SARS-CoV-2 belongs to the family of β-coronavirus. Its genome is about 30 kb long and shares about 80% of genome identity with SARS-CoV. The 3’ proximal terminal consists of 4 structural proteins: Spike (S), Membrane (M), Envelope (E), and Nucleocapsid (N). The S protein is a homo-trimeric, class I fusion, transmembrane glycoprotein. It promotes attachment, fusion, and entry of the virus into the host cells. The protein consists of two functional subunits, S1 and S2. The S1 subunit contains the N-terminal domain (NTD) and the C-terminal domain (CTD). The C-terminal domain consists of the receptor-binding domain (RBD) that binds to the angiotensin-converting enzyme 2 (ACE2) receptor located on the host cells. Given the critical role of RBD in viral entry into the host cell, it could serve as an efficient target antigen for the development of vaccines. BioVision’s SARS-CoV-2 RBD ELISA Kit is designed to quantitatively measure the amount of RBD protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples. The assay is based on the Sandwich ELISA principle. Test samples, Standards, and Biotin-conjugated antibody are added to the wells pre-coated with the RBD antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of RBD in the sample or standard.
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