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Product Details
| Cat # +Size | E4821-100 |
|---|---|
| Size | 96 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Human |
| Applications | ELISA kit for the qualitative determination of SARS-CoV-2 Nucleoprotein IgG antibody concentrations in serum and plasma samples.• |
| Features & Benefits | • Easy, convenient, sensitive and time-saving method to qualitative determination of SARS-CoV-2 Nucleoprotein IgG antibody concentrations in serum and plasma samples.
• ntra-assay Precision: CV% <15%; Inter-assay Precision: CV% <15% |
| Kit Components | • Micro ELISA Plate
• Negative Control • Positive Control • HRP Conjugate (100x) • HRP Conjugate Diluent • Sample Diluent • Wash Buffer (25x) • TMB Substrate • Stop Solution • Plate sealers |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Severe acute respiratory syndrome (SARS) is a viral respiratory illness caused by a coronavirus called SARS-associated coronavirus (SARS-CoV). The coronavirus genome encodes a spike protein (S), an envelope protein, a membrane protein, and a nucleoprotein in this order. Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. BioVision’s SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit is based on indirect qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SARS-CoV-2 Nucleoprotein. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 Nucleoprotein IgG antibody bound in the initial step. The color development is stopped by adding stop solution and the intensity of the color is measured.
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