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SARS-CoV-2 Nucleocapsid ELISA Kit

A Sandwich ELISA kit to Quantitatively measure Nucleocapsid protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4878
$695.00

Product Details

Cat # +Size E4878-100
Size 96 assays
Detection Method Absorbance (450 nm)
Applications This Sandwich ELISA is highly sensitive and highly specific for the detection of Nucleocapsid protein in bronchoalveolar lavage fluid and nasopharyngeal samples
Features & Benefits • This Sandwich ELISA is highly sensitive and highly specific for the detection of Nucleocapsid protein in bronchoalveolar lavage fluid and nasopharyngeal samples
• Assay Precision: Intra-Assay CV < 12% and Inter-Assay CV < 15%
• Sensitivity: < 1 ng/ml
• Detection range: 12.5 – 80 ng/ml
Kit Components • Capture ELISA plate
• SARS-CoV-2 NP Standard (160 ng)
• Standard/Sample Dilution Buffer
• NP Biotin Conjugated Antibody
• Reagent Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (20X)
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Coronaviruses are a diverse group of RNA viruses that infect humans, mammals, and avian species. Previously there were seven known human coronaviruses, of which two were considered highly pathogenic and caused fatal respiratory illness, namely, SARS-CoV and MERS-CoV. A novel SARS-CoV-2 virus emerged at the end of 2019 that resulted in COVID-19 disease, which proved to be deadlier than its predecessors and resulted in a worldwide pandemic. SARS-CoV-2 is a large single-stranded, positive-sense RNA that is genetically similar to SARS-CoV (80%). It consists of 4 structural proteins required for viral assembly: Spike (S), Envelope (E), Membrane (M), and Nucleocapsid (N) protein. The Nucleocapsid is the most abundant protein in SARS-CoV-2. Due to its critical role in viral assembly, the Nucleocapsid protein could serve as a potential target for vaccine and drug treatments. BioVision’s SARS-CoV-2 Nucleocapsid ELISA Kit is designed to quantitatively measure the amount of Nucleocapsid proteins in bronchoalveolar lavage fluid and nasopharyngeal swab samples. The assay is based on the Sandwich ELISA principle. Test samples, Standards, and Biotin-conjugated antibody are added to the wells pre-coated with Nucleocapsid antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of Nucleocapsid in the sample or standard.


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