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Sarcosine Colorimetric/Fluorometric Assay Kit

based on 2 citations in multiple journalsSarcosine Colorimetric/Fluorometric Assay Kit24.1 4
Catalog #: K636

In stock

$365.00

Product Details

Cat # +Size K636-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The kit can detect Sarcosine in the detection range of 1 – 10000 µM.
Features & Benefits • Simple procedure; takes ~ 1 hour
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Sarcosine
Kit Components • Sarcosine Assay Buffer
• Sarcosine Probe (in DMSO, anhydrous)
• Sarcosine Enzyme mix
• Sarcosine Standard (10 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sarcosine, a natural amino acid, plays important roles as intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine, etc. Detection of sarcosine level has wide applications in research and development. BioVision’s Sarcosine Assay Kit provides an accurate, convenient measure of sarcosine in variety biological samples. In the assay, sarcosine is specifically oxidized to generate a product that converts a colorless probe to a product with intense red color (λmax = 570 nm) and which is also highly fluorescent (Ex/Em = 538/587 nm). Sarcosine is therefore easily detected by either colorimetric or fluorometric methods with detection range 1 – 10000 µM.


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What is the detection range of this kit and what is the normal amounts found in humans?
Sarcosine detection range is 1 – 10000 µM. 1. The concentration of sarcosine in blood serum of normal human subjects is 1.59 ± 1.08 nanomolar. http://www.rsc.org/learn-chemistry/resource/rws00001057/sarcosine?cmpid=CWS00001057
What is the volume of human urine sample required for this assay?
We do not suggest using urine samples with this kit since there is a lot of interfering substances in urine.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can alternate buffers be used for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Is it essential to make a standard curve for every expt, or is one curve/kit enough?
Yes, it is strongly recommended to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Ferro et al., Improving the Prediction of Pathologic Outcomes in Patients Undergoing Radical Prostatectomy: The Value of Prostate Cancer Antigen 3 (PCA3), Prostate Health Index (Phi) and Sarcosine. Anticancer Res, Feb 2015; 35: 1017 - 1023.
Biavardi, E. et al., Exclusive recognition of sarcosine in water and urine by a cavitand-functionalized silicon surface PNAS, Feb 2012; 109: 2263 - 2268.
For more citations of this product click here