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Sarafloxacin ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Sarafloxacin concentrations in Tissue, Honey, Milk, Egg, Urine
Catalog #: E4777

Product Details

Cat # +Size E4777-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications In vitro quantitative determination of Sarafloxacin
Features & Benefits • Cross-reactivity: Sarafloxacin -100%,
• Detection limit: Tissue (chicken, pork, fish, shrimp) - 0.3 ppb, Honey - 0.4 ppb, Milk - 3 ppb, Milk powder- 6 ppb, Egg - 3 ppb, Urine - 0.5 ppb
• Cross-reactivity: Sarafloxacin -100%,
• Sensitivity: 0.1 ppb (ng/mL)
• Sample recovery rate: Tissue, Honey, Milk, Milk powder, Egg- 85%±15%
Kit Components • HRP Conjugate
• Stop Solution
• Substrate Reagent B
• ELISA plate
• Plate Sealer
• Standard
• Antibody Working Solution
• Reconstitution Buffer
• Wash Buffer (20X)
• Substrate Reagent A
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Sarafloxacin is a veterinary fluoroquinolone antimicrobial agent that inhibits bacterial DNA gyrase. It has been used for the control of bacterial infections in poultry caused by either E. coli or Salmonella species. Sarafloxacin ELISA Kit is based on the Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Sarafloxacin. During the reaction, Sarafloxacin in the samples or standard competes with Sarafloxacin coated on the plate for binding to the anti-Sarafloxacin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sarafloxacin. The concentration of Sarafloxacin in the samples can be calculated by comparing the OD of the samples to the standard curve.

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