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S-Adenosylmethionine (SAM) ELISA Kit

A competitive ELISA kit for the quantitative measurement of S-Adenosylmethionine in serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4541

In stock

$695.00

Product Details

Cat # +Size E4541-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of SAM in serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications Quantitative measurement of SAM in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of SAM in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.39 – 25 μg/ml
• Sensitivity: < 0.234 μg/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• No significant cross-reactivity or interference with its analogues was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

S-Adenosylmethionine (SAM) is a common cosubstrate involved in methyl group transfers, transsulfuration, and aminopropylation. Although these anabolic reactions occur throughout the body, most SAM-e is produced and consumed in the liver. In bacteria, SAM-e is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis. In eukaryotic cells, SAM-e serves as a regulator of a variety of processes including DNA, tRNA, and rRNA methylation; immune response; amino acid metabolism; transsulfuration; and more. In plants, SAM-e is crucial to the biosynthesis of ethylene, an important plant hormone and signaling molecule. BioVision’s SAM ELISA kit is a sandwich ELISA assay for the quantitative measurement of SAM in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of SAM captured from the samples.


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