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RTEL1 (Rat) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of RTEL1 in Rat plasma, tissue homogenates and other biological fluids.
Catalog #: E4812
$695.00

Product Details

Cat # +Size E4812-100
Size 96 assay
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Rat
Applications • A Sandwich ELISA kit for the quantitative measurement of RTEL1 in Rat plasma, tissue homogenates, and other biological fluids.
Features & Benefits • Sensitivity: 46.875 pg/ml
• Detection Range: 78.125-5000 pg/ml
Kit Components • Micro ELISA Plate
• Lyophilized Standard (5000 pg)
• Sample / Standard dilution buffer
• Biotin- labeled antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Regulator of telomere elongation helicase 1 is an ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. It acts as an anti-recombinase to counteract toxic recombination and limits crossover during meiosis. A 96-well plate was coated with a capture antibody. BioVision’s RTEL1 (Rat) ELISA Kit is based on the Sandwich ELISA principle. A 96-well ELISA microplate was coated with capture antibody the biotin-conjugated antibody was used as a detection antibody. The standards, test samples, and biotin conjugated detection antibody were added to the wells subsequently and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding the acidic stop solution. The density of color is proportional to the amount of RTEL1 captured from the samples.


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