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ROS1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of ROS1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5004
$795.00

Product Details

Cat # +Size E5004-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of ROS1 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
● Sensitivity: 18.75 pg/ml
● Detection range: 31.25 – 2000 pg/ml
● This Sandwich ELISA is highly sensitive and highly specific for the detection of ROS1 in human samples. There is no significant cross-reactivity or interference between ROS1 and analogues
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (2000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

ROS1 is an orphan receptor tyrosine kinase that belongs to the family of insulin receptors. This protein has tyrosine kinase activity and is structurally similar to anaplastic lymphoma kinase (ALK). The exact biological function of ROS1 remains unknown; however, studies have demonstrated that chromosomal rearrangement results in ROS1 fusion that promotes oncogenesis in non-small cell lung cancer, gastric, colorectal, ovarian cancer, and glioblastoma. Multiple drugs such as Crizotinib, Ceritinib, and Entrectinib target ROS1 fusion in cancers and are currently under investigation in clinical trials. BioVision’s ROS1 (Human) ELISA kit quantitatively measures ROS1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ROS1 in the sample or standard.


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