Product Details
| abID | ab273299 |
|---|---|
| Cat # +Size | K934-100 |
| Size | 100 assays |
| Kit Summary | BioVision’s RNAse Activity Detection Assay Kit enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time. |
| Detection Method | Fluorescence (Ex/Em = 495/520 nm) |
| Species Reactivity | All |
| Applications | • Quantitative analysis of RNAse activity of purified enzymes • Measurement of RNAse contamination in buffers and samples |
| Features & Benefits | • Highly sensitive • Specific • The limit of quantification is 1.2 pg RNase/well • The limit of detection is 0.4 pg RNase/well • High Throughput compatible |
| Kit Components | • Molecular Biology Grade Water • RNase Positive Control • Half-area Plate • 10X RNase Buffer • Fluorescence Standard (10 mM in DMSO) • RNase Probe |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Many molecular biology applications require RNase-free environment due to the degradation of RNA samples by RNase. Obtaining full length, high quality RNA samples is essential, yet challenging in genomics settings. BioVision’s RNAse Activity Detection Assay Kit enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time. The assay uses a highly sensitive, specific probe that releases a fluorescent product in the presence of active RNAse. The limit of detection is 0.4 pg RNase/well and limit of quantification is 1.2 pg RNase/well.
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