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RNase A, Recombinant

Animal Origin Free, DNAse and Protease free
Catalog #: M1517
SKU-Size Size Price Qty
M1517-25 25 mg
$195.00
M1517-50 50 mg
$295.00
More Sizes Get Quote

Product Details

Highlights ● Highly stable
● DNase, protease and endotoxin free
● For RNase protection assays and purification of DNA/plasmid
● Animal Origin free
Form Lyophilized powder
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

RNase A is an endoribonuclease that specifically cleaves the single strand RNA at the phosphodiester bond between the 5’-ribose of a nucleotide and the 3’ OH phosphate group of an adjacent pyrimidine nucleotide. The highest activity is demonstrated with single stranded RNA. The recombinant enzyme is identical to the native RNase A in amino acid sequence, structure and specifications. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA. Precipitation may occur at high concentrations (>10 mg/ml) of the enzyme, The enzyme is inhibited by diethyl pyrocarbonate (DEPC), guanidinium salts, β-mercaptoethanol, heavy metals and RNase-inhibitors. The RNase A has a high affinity to glass surfaces.


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