Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Ribavirin ELISA Kit

A Competitive ELISA kit for the quantitative determination of Ribavirin in tissue, milk, and egg samples

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California
to cause cancer. For more information go to www.p65warnings.ca.gov/.
Catalog #: E4949
$695.00

Product Details

Cat # +Size E4949-100
Size 96 assays
Kit Summary Ribavirin is a guanosine analog and an anti-viral agent that interferes with viral mRNA synthesis and capping. Upon activation by adenosine kinase, ribavirin triphosphate binds to the nucleotide-binding site of the viral mRNA polymerase, thereby preventing the binding of correct nucleotides and causing decreased viral replication and formation of defective virions. Ribavirin also inhibits viral mRNA guanylyltransferase and 2′-O-methyltransferase, thereby disrupting the posttranslational capping of the viral mRNA. The drug is recommended to treat hepatitis C and viral hemorrhagic fevers. BioVision’s Ribavirin ELISA kit is used to quantitatively measure Ribavirin in muscle tissue, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Ribavirin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Ribavirin in the samples.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications Competitive ELISA kit to quantitatively determine Ribavirin in tissue, milk, and egg samples
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of Ribavirin
• Cross reaction: Ribavirin 100%
• Sensitivity: 0.2ppb
• Detection Limit: 8ppb for muscle tissue, 2ppb for raw milk, 4ppb for egg
Kit Components • Micro ELISA Plate Plate Sealer
• Standard (S0 – S5)
• HRP Conjugate (11X)
• HRP Conjugate diluent
• Substrate A
• Substrate B
• Stop Solution
• Wash Buffer (20X)
• Sample diluent
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Ribavirin is a guanosine analog and an anti-viral agent that interferes with viral mRNA synthesis and capping. Upon activation by adenosine kinase, ribavirin triphosphate binds to the nucleotide-binding site of the viral mRNA polymerase, thereby preventing the binding of correct nucleotides and causing decreased viral replication and formation of defective virions. Ribavirin also inhibits viral mRNA guanylyltransferase and 2′-O-methyltransferase, thereby disrupting the posttranslational capping of the viral mRNA. The drug is recommended to treat hepatitis C and viral hemorrhagic fevers. BioVision’s Ribavirin ELISA kit is used to quantitatively measure Ribavirin in muscle tissue, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Ribavirin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Ribavirin in the samples.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.