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Renin (Human) ELISA Kit

A Sandwich ELISA kit applies for in vitro quantitative determination of Human REN concentrations in serum, plasma and other biological fluids.
Catalog #: E4719
$525.00

Product Details

Cat # +Size E4719-100
Size 100 assays
Detection Method Absorbance-450 nm
Species Reactivity Human
Applications • Detection Range: 31.25—2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Specificity: This ELISA kit recognizes Human REN in samples. No significant cross-reactivity or interference between Human REN and analogues was observed.
Features & Benefits • Detection Range: 31.25—2000 pg/mL
• Specificity: This ELISA kit recognizes Human REN in samples. No significant cross-reactivity or interference between Human REN and analogues was observed.
• Sensitivity: 18.75 pg/mL
Kit Components • Biotinylated Detection Anybody Diluent
• Wash Buffer (25x)
• HRP Conjugate (100x)
• Micro ELISA Plate
• Substrate Reagent
• Biotinylated Detection Antibody (100x)
• Stop Solution
• Standard
• HRP Conjugate Diluent
• Plate Sealer
• Standard & Sample Diluent
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. BioVision's Renin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each microplate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human REN. You can calculate the concentration of Human REN in the samples by comparing the OD of the samples to the standard curve.


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