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Red Fluorescent Protein - R-PE (R-Phycoerythrin)

Fluorescent protein which can be cross linked or conjugated to other molecules to make fluorescent probes.
Catalog #: 6005
SKU-Size Size Price Qty
6005-1 1 mg
$75.00
6005-5 5 mg
$195.00
More Sizes Get Quote

Product Details

Alternate Name RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Gene Symbol R-PE
Source Febico’s proprietary red algae
Appearance Suspension
Physical Form Description R-PE is supplied in 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.
Molecular Weight 240 kDa
Purity by SDS-PAGE A566/A280 ≥ 5.5
Purity by A566/A498 ≤1.5
Reconstitution Instructions Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tu
Handling Centrifuge the vial prior to opening.
Storage Conditions 2-8°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.


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