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Product Details
| Alternate Name | RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin |
|---|---|
| Gene Symbol | R-PE |
| Source | Febico’s proprietary red algae |
| Appearance | Suspension |
| Physical Form Description | R-PE is supplied in 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide. |
| Molecular Weight | 240 kDa |
| Purity by SDS-PAGE | A566/A280 ≥ 5.5 |
| Purity by | A566/A498 ≤1.5 |
| Reconstitution Instructions | Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tu |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | 2-8°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.
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