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Recombinant hSUMO1-AMC

Pure and sensitive AMC tagged fluorogenic substrate for deSUMOylating enzymes.
Catalog #: 6412
$455.00

Product Details

Cat # +Size 6412-50
Size 50 μg
Gene Symbol SUMO1
Gene ID 7341
Accession # P63165
Appearance Lyophilized powder
Physical Form Description Lyophilized powder
Molecular Weight 11.142 kDa
Purity by SDS-PAGE ≥95%
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.


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