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Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1

based on 1 citations in multiple journalsReady-to-use Cell Proliferation Colorimetric Reagent, WST-114.1 4
Sensitive, Non-radioactive Assay
Catalog #: K304

Product Details

Cat # +Size K304-2500
Size 2500 assays
Detection Method Absorbance 440 nm
Species Reactivity Mammalian
Applications Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc.
Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
Assessment of physiological mediators and antibodies that inhibit cell growth.
Features & Benefits • Simple one-step procedure; takes less than 4 hours
• Fast and convenient
• The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
Kit Components • WST-1 Reagent (in electron coupling solution)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.

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Why do we need to set the reference wavelength at 650nm? Is that necessary for this specific colorimetric assay?
For colorimetric assays, the purpose of using a reference wavelength is to correct/normalize any change that is not from the OD of your analyte (formazan). If your 650 nm OD is high, it means that it needs to be subtracted from OD measured at 440 nm (assuming this is the filter that you used for your samples). It is more like a background reading of your samples.
What is the reason for reading the signal at 2 wavelength, OD 420 and 480nm?
This does not mean you read OD at 2 wavelengths. This means that you can read the OD anywhere between 420 nm - 480 nm wavelengths. You can choose any filter that is between 420 nm and 480 nm. If you choose 440 nm filters, then you run your samples twice – one at 440 nm and one at your reference wavelength, which is at 650 nm.
Tu, Dom-Gene et al. (2016) Hinokitiol inhibits vasculogenic mimicry activity of breast cancer stem/progenitor cells through proteasome‑mediated degradation of epidermal growth factor receptor, Oncol Lett. 2016 Apr;11(4):2934-2940.
Cheng et al., Autophagy modulates endoplasmic reticulum stress-induced cell death in podocytes: A protective role. Experimental Biology and Medicine, Apr 2015; 240: 467 - 476.
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