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Reactive Oxygen Species (ROS) Detection Assay Kit

Highly Sensitive Assay that measures Reactive Oxygen Species. Non-radioactive, High-Throughput
Catalog #: K936
SKU-Size Size Price Qty
K936-100 100 assays
K936-250 250 assays
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Product Details

Kit Summary • Detection method- Fluorescence (Ex/Em = 495/529 nm).
• Applications- Measurement of intracellular levels of ROS
• Screening compounds with a stress inducing and/or antioxidant function.
Detection Method Fluorescence (Ex/Em = 495/529 nm).
Species Reactivity Eukaryotes
Applications • Measurement of intracellular levels of ROS.
• Screening compounds with a stress inducing and/or antioxidant function
Features & Benefits • Simple & Rapid Protocol
• Convenient: Non-Radioactive, no special handling or disposal required
• High-Throughput
• Accurate: reproducible results with low intra & inter assay variability
Kit Components • ROS Assay Buffer
• ROS Label (1000X)
• ROS Inducer (250X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells. Low levels of ROS play an essential role in signaling pathways, whereas increased under oxidative stress, ROS activity result in damage to nucleic acids, proteins and membrane lipids. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as; vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. BioVision’s ROS Detection Assay Kit is designed for detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as a control for measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy

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I want to do several days of treatment with a drug. Can I pretreat the cells rather than labeling first and then treatment?
We would not recommend this for two reasons. 1) If you treat your cells for several days with a ROS inducer, cell's native mechanism is going to induce anti-oxidant generation to counter balance the oxidative stress. 2) If you pre-treat your cells with a drug, all the ROS generated by the drug will not be accounted for in the oxidation of the H2DCF, when H2DCF is added later. Some of the ROS generated will be already negated by the anti-oxidants generated by the cells.
Can we use this kit to measure ROS in serum or plasma?
K936 is a cell based assay and is used for real-time measurement of global levels of ROS in living cells. The assay is optimized for adherent cells or suspension cells. Thus, it is not recommended for serum or plasma samples.
Sun Kyung Kim. Identification of drug combinations on the basis of machine learning to maximize anti-aging effects. PLoS One. Jan 2021. 33507975.
Michael M Farag. Hyaluronic Acid Conjugated Metformin-Phospholipid Sonocomplex: A Biphasic Complexation Approach to Correct Hypoxic Tumour Microenvironment. Int J Nanomedicine. Feb 2021. 33603365.
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