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Raf1 Antibody

based on 1 citations in multiple journalsRaf1 Antibody14.1 4
Catalog #: 3116
SKU-Size Size Price Qty
3116-30T 30 µg
3116-100 100 µg
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Product Details

Antibody Target Raf1
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Synthetic peptide mapping to the C-terminus of human Raf-1
Accession # P04049
Gene ID 5894
Appearance Colorless liquid
Concentration 0.2 mg/ml
Formulation 100 µg (0.2 mg/ml) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification Affinity purified
Species Reactivity Human, mouse, rat
Application Western blot, Immunoprecipitation, Immunohistochemistry
Application & Usage Western blot analysis (1-4 µg/ml), immunoprecipitation (15-25 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.

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Ito J-I et al. (2007) J. Lipid Res. 48: 2020 -2027
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