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Ractopamine ELISA Kit

A competitive ELISA kit for the quantitative measurement of Ractopamine in urine, pork, liver, feed

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4565

Product Details

Cat # +Size E4565-100
Size 96 assays
Kit Summary Quantitative measurement of Ractopamine in tissue, feed, liver, urine.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for in vitro quantitative determination of Ractopamine concentrations in tissue, feed, liver, urine.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Ractopamine in tissue, feed, liver, urine.
• Detection Range: 0.1 ppb-8.1 ppb
• Sensitivity: 0.1 ppb.
• Detection limitation: 0.3 ppb for urine, 0.4 ppb for tissue, 2 ppb for liver, 2 ppb for feed
• Cross Reactivity: Ractopamine (RA) 100%, Salbutamol (SALB) <0.1%, Dobutamine <1%, Clenbuterol <0.1%
Kit Components • Micro ELISA Plate
• Standard (S0 – S5)
• HRP-conjugate
• Antibody
• Substrate A
• Substrate B
• Stop Solution
• Sample Ressolving (10×)
• Wash Buffer (20×)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Ractopamine (RA) is a synthetic beta-adrenoreceptor agonist. It is a phenol-based TAAR1 agonist and β adrenoreceptor agonist that stimulates β1 and β2 adrenergic receptors. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 0.1 ppb, and linear range from 0.1 ppb to 8.1 ppb. Ractopamine ELISA Kit is designed for the quantitative determination of Ractopamine concentrations in tissue, feed, liver, urine. The kit is based on the Competitive ELISA principle. The microtiter plate provided in this kit has been pre-coated with Ractopamine antigen. Standards or samples are added to the appropriate microtiter plate wells with Ractopamine specific antibody. The competitive inhibition reaction is launched between pre-coated Ractopamine and Ractopamine in standards or samples with the Ractopamine special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Ractopamine in the standards or samples. The color development is stopped and the intensity of the color is measured.

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