R-Spondin-2, human recombinant

Regulates the proliferation and differentiation of certain stem cell populations
Catalog #: 7190 | abID:

Product Details

Alternate Name Roof plate-specific Spondin 2, Rspo2
Gene Symbol RSPO2
Gene ID 340419
Accession # Q6UXX9
Source CHO Cells
Appearance Lyophilized powder
Physical Form Description Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.
Molecular Weight 30.0 kDa
Purity by SDS-PAGE ≥95%
Endotoxin Level < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity R-Spondin-2 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri
Amino Acid Sequence ASYVSNPICK GCLSCSKDNG CSRCQQKLFF FLRREGMRQY GECLHSCPSG YYGHRAPDMN RCARCRIENC DSCFSKDFCT KCKVGFYLHR GRCFDECPDG FAPLEETMEC VEGCEVGHWS EWGTCSRNNR TCGFKWGLET RTRQIVKKPV KDTILCPTIA ESRRCKMTMR HCPGGKRTPK AKEKRNKKKK RKLIERAQEQ HSVFLATDRA NQ
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-2 is a 24.4 kDa protein consisting of 212 amino acid residues. Due to glycosylation, R-Spondin-2 migrates at an apparent molecular weight of approximately 30.0 kDa by SDS PAGE analysis under reducing conditions.


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