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QuickDetect™ Pyruvate Kinase (Human) ELISA Kit

A rapid sandwich ELISA kit for the quantitative measurement of Pyruvate Kinase in human serum, plasma, urine, cell culture samples and other biological fluid

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4448

Product Details

Cat # +Size E4448-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- human
• Application- quantitative measurement of PK in serum, plasma, urine, cell culture samples, tissue samples
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of total PK in human serum, plasma, urine, cell culture samples, biological fluid.
Features & Benefits • Easy, convenient and time-saving method to measure the level of PK in human Serum, plasma, urine, cell culture samples, biological fluid.
• Detection Range: 8 - 500 pg/ml
• Sensitivity: < 2.2 pg/ml
• Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Kit Components • Micro ELISA strip-plate
• Standard
• Standard diluent
• HRP- Conjugate reagent
• Sample diluent
• Chromogen Solution A
• Chromogen Solution B
• Stop Solution
• Wash buffer (30X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Pyruvate kinase is the enzyme that catalyzes the final step of glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP), yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase is present in four distinct, tissue-specific isozymes, each consisting of particular kinetic properties necessary to accommodate the variations in metabolic requirements of diverse tissues. BioVision’s PK ELISA kit is a sandwich ELISA assay for the quantitative measurement of PK in human serum, plasma and cell culture supernatants in 90 minutes. The density of color is proportional to the amount of PK captured from the samples.

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