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QuickDetect™ Alpha-enolase (ENO1) (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Alpha-enolase in human serum, plasma, culture media samples
Catalog #: E4706

In stock

$495.00

Product Details

Cat # +Size E4706-100
Size 100 assays
Detection Method Absorbance-450 nm
Species Reactivity Human
Applications • Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%, CV (%) = SD/mean X 100
• Sensitivity: 0.1 ng/mL
• Detection of Alpha-enolase in human serum, plasma, culture media samples.
• Detection range: 1.25 ng/mL - 40 ng/mL
Features & Benefits • Sensitivity: 0.1 ng/mL
• Detection range: 1.25 ng/mL - 40 ng/mL
• Detection of Alpha-enolase in human serum, plasma, culture media samples.
Kit Components • Micro Elisa strip plate
• Standard diluent
• Chromogen Solution A
• Sample diluent
• HRP-Conjugate reagent
• Chromogen Solution B
• Wash solution (20X)
• Standard:80 ng/mL
• Stop Solution
• Plate sealers
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

BioVision's α-enolase ELISA kit is based on Sandwich ELISA method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to ENO1. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP) - conjugated antibody specific for ENO1 is added to each Micro Elisa strip plate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain ENO1 and HRP conjugated ENO1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ENO1. The concentration of ENO1 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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