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Pyruvate Carboxylase Activity Assay Kit (Colorimetric)

To measure pyruvate carboxylase activity in various samples
Catalog #: K2075
$515.00

Product Details

Cat # +Size K2075-100
Size 100 assays
Kit Summary BioVision’s Pyruvate Carboxylase Activity Assay Kit provides a facile, rapid way to measure PC activity in various biological samples.
Detection Method Absorbance at 340 nm
Species Reactivity Mammalian
Applications Measurement of pyruvate carboxylase activity in various samples.
Features & Benefits • The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples.
• A facile, rapid way to measure pyruvate carboxylase activity in various biological samples.
Kit Components • PC Assay Buffer
• PC Substrate I
• PC Substrate II
• PC Cofactor
• PC Developer
• NADH Standard
• PC Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Pyruvate carboxylase (PC) catalyzes the irreversible carboxylation of pyruvate to oxaloacetate (OAA), depending on the species. It is a tetramer composed of four identical monomers each with a molecular weight of 125 kDa. OAA produced by PC is an important intermediate in numerous biological pathways. In mammals, PC plays an essential role in gluconeogenesis, lipogenesis, synthesis of neurotransmitters, and glucose-induced insulin secretion by pancreatic islets. During nutritional overload, PC levels are increased in the pancreatic β-cells to increase pyruvate cycling due to an increase in glucose levels. PC deficiency can lead to lactic acidosis due to the accumulation of lactic acid. BioVision’s Pyruvate Carboxylase Activity Assay Kit provides a facile, rapid way to measure PC activity in various biological samples. In this kit, pyruvate is carboxylated to OAA, which is further detected by the oxidation of NADH. Therefore, a decrease in NADH absorbance at 340 nm is proportional to the amount of PC in samples. The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples. Google Description: Pyruvate carboxylase (PC) catalyzes the irreversible carboxylation of pyruvate to oxaloacetate (OAA), depending on the species. It is a tetramer composed of four identical monomers each with a molecular weight of 125 kDa. OAA produced by PC is an important intermediate in numerous biological pathways. In mammals, PC plays an essential role in gluconeogenesis, lipogenesis, synthesis of neurotransmitters, and glucose-induced insulin secretion by pancreatic islets. During nutritional overload, PC levels are increased in the pancreatic β-cells to increase pyruvate cycling due to an increase in glucose levels. PC deficiency can lead to lactic acidosis due to the accumulation of lactic acid. BioVision’s Pyruvate Carboxylase Activity Assay Kit provides a facile, rapid way to measure PC activity in various biological samples. In this kit, pyruvate is carboxylated to OAA, which is further detected by the oxidation of NADH. Therefore, a decrease in NADH absorbance at 340 nm is proportional to the amount of PC in samples. The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples.


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