Pyrophosphate (PPi) Assay Kit(Fluorometric/Colorimetric)

Simple, Highly Sensitive, HTS adaptable assay to measure Pyrophosphate in a variety of biological samples.

K568 is available from Abcam as ab234040.
Catalog #: K568 | abID: ab234040

Product Details

abID ab234040
Cat # +Size K568-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em = 535/587 nm) or Absorbance (OD 570 nm)
• Species reactivity- Mammalian
• Applications- Estimation of Pyrophosphate in various biological samples.
• Detection limit/sensitivity: Can detect as little as 2 µM of Pyrophosphate
Detection Method Detection method- Fluorescence (Ex/Em = 535/587 nm) or Absorbance (OD 570 nm)
Species Reactivity Eukaryotes
Applications • Determination of PPi concentration in biological samples
• Monitor pyrophosphate release by a variety of enzymes
• Screening inhibition or activities of enzymes that consume pyrophosphate
Features & Benefits • Simple, sensitive and HTP adaptable Protocol
• Specific for Pyrophosphate: other molecules containing phosphate groups do not interference with the assay
• This kit can detect as little as 2 µM of Pyrophosphate in a variety of biological samples
Kit Components • PPi Assay Buffer
• PPi Buffer Supplement
• PPi Substrate
• PPi Enzyme Mix
• PPi Developer
• PPi Probe
• PPi Standard (1 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Inorganic pyrophosphate (diphosphate, pyrophosphoric acid or PPi) is a small molecule formed during, or required as a substrate for, a number of biochemical reactions. Enzymatic reactions that generate PPi include ATP hydrolysis, DNA and RNA polymerization, cyclic AMP formation, and enzymatic activation of fatty acids to form their CoA esters. Conversely, enzymes that utilize PPi may include cyclases, hydrolases and ligases. Measurement of inorganic pyrophosphate (PPi) in plasma, serum and other biologic fluids is important in studies of bone metabolism, renal stone disease, and certain types of arthritis. BioVision’s Pyrophosphate (PPi) Assay Kit provides a fast, convenient and ultrasensitive method for determination of free inorganic pyrophosphate levels in biological material. PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (Ex/Em = 535/587 nm) or color (OD: 570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay. Our assay delivers an easy and robust method suitable for use in a variety of biological samples and can be performed in a convenient microtiter-plate format. The kit provides sufficient reagents for 100 fluorometric or 50 colorimetric assays, respectively. This kit can detect as low as 1.8 μM PPi in plasma and serum samples.

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Could you please share the principle of the assay? Will ATP contamination of my samples interfere with the assay?
PPi present in the cells/tissues is converted through series of enzymatic reactions generating a fluorometric or colorimetric end product, which is then detected to quantify the amount of PPi. ATP, ADP or monomeric inorganic phosphate (Pi) will not interfere with the assay.