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Puromycin Dihydrochloride

A protein synthesis inhibitor
Catalog #: 1860
SKU-Size Size Price Qty
1860-25 25 mg
$70.00
1860-100 100 mg
$205.00
1860-250 250 mg
$375.00
1860-500 500 mg
$695.00
1860-1000 1 g
$1,145.00
More Sizes Get Quote

Product Details

Alternate Name 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
Appearance White to off-white solid
CAS # 58-58-2
Molecular Formula C₂₂H₂₉N₇O₅ · 2HCl
Molecular Weight 544.43
Purity ≥98% by HPLC
Solubility H₂O (~50 mg/ml)
SMILES CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
InChi InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H
InChi Key MKSVFGKWZLUTTO-UHFFFAOYSA-N
PubChem CID 16757772
MDL Number MFCD00012691
Handling Protect from air and moisture
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

A nucleoside antibiotic. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 µg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 µg/ml.


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What is the recommended method of reconstitution? Once dissolved, what temperature should it be stored at and for how long can it be stored this way?
You can reconstitute Puromycin Dihydrochloride in water. Once reconstituted, it should be aliquoted abd stored at -20˚C in dark.
Yan Qiu. Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology. STAR Protoc. Jan 2021. 33554142.