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PTGS2/COX-2 (Mouse) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of PTGS2/COX-2 in human serum, plasma, culture media samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4800
$695.00

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity Mouse
Applications in vitro quantitative measurement of PTGS2/COX-2 in human serum, plasma, culture media samples.
Features & Benefits • Specificity:No Significant cross-reactivity or interference between Mouse PTGS2/COX-2 and analogues was observed.
• Precision: Coefficient of variation is < 10%.
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.10 ng/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The prostaglandin-endoperoxide synthase 2 (COX-2)] is an enzyme induced by proinflammatory stimuli that is often overexpressed in malignant tissue and involved in the synthesis of prostaglandins and thromboxanes, regulators of processes such as inflammation, cell proliferation, and angiogenesis, all relevant for cancer development. BioVision’s PTGS2/COX-2 (Mouse) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of PTGS2/COX-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse PTGS2/COX-2. The concentration of Mouse PTGS2/COX-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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