Protein L

Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Catalog #: 6530 | abID: ab286201

Product Details

abID ab286201
Alternate Name Protein L
Accession # CAA25612
Source E. coli
Appearance Lyophilized
Physical Form Description Lyophilized with no additives
Molecular Weight 43.2 kDa with 5 IgG binding sites
Purity by SDS-PAGE ≥98%
Endotoxin Level <0.1 ng/μg
Reconstitution Instructions Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans


Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
Can Protein L bind to λ-chains?
As far as we know, Protein L binds Kappa I, III and IV in humans and Kappa I on mouse. Protein L is not a universal antibody-binding protein and is restricted to those antibodies that contain kappa light chains.
Hiroyasu Muramatsu. Novel myostatin-specific antibody enhances muscle strength in muscle disease models. Sci Rep. Jan 2021. 33495503.
For more citations of this product click here