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Protein L Magnetic Beads

Reusable Protein L conjugated magnetic beads with ≥10 mg/ml binding capacity for antibody purification from multiple sources
Catalog #: 6537

Product Details

Cat # +Size 6537-1
Size 1 ml
Highlights • CONTENTS:
Supplied as a 50% slurry in PBS with 0.02% sodium azide.
Support Characteristics- Paramagnetic, spherical, 4 % cross-linked agarose
Ligand- Recombinant Protein L
Particle Size- 75 – 150 µm
Binding Capacity- Generally >10 mg human IgG/ml wet beads
Working Temperature- Room temperature
Storage Solution- PBS with 0.02% NaN₃
• FEATURES: Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of antibodies. High affinity for kappa-light chain containing Ig antibodies from a variety of species. Protein L binds to all IgG subclasses from human, mouse and rat species. It also binds to human, mouse and rat IgM, IgA, IgE and IgD, as well as FAB with Kappa-light chains. Protein L is superior for binding chicken, hamster and pig IgG.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein L Magnetic Beads are prepared by covalently coupling Recombinant Protein L (contains five Ig kappa light chain binding domains, BV catalog # 6530) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per ml of wet gel.

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Can the Protein L magnetic beads be re-used?
6537-1 Protein L magnetic beads could be re-used. However, we recommend re-using it with the same sample type to avoid possible cross contamination. We recommend using the below protocol for regeneration:
Regenerate the column by:
a. Washing with ~ 5 volumes of Elution Buffer.
b. Equilibrate with 5 volumes of Binding Buffer containing 0.2% sodium azide.
c. Store upright at 4 °C.