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Product Details
| Cat # +Size | 6537-1 |
|---|---|
| Size | 1 ml |
| Highlights | • CONTENTS: Supplied as a 50% slurry in PBS with 0.02% sodium azide. • TECHNICAL SPECIFICATIONS: Support Characteristics- Paramagnetic, spherical, 4 % cross-linked agarose Ligand- Recombinant Protein L Particle Size- 75 – 150 µm Binding Capacity- Generally >10 mg human IgG/ml wet beads Working Temperature- Room temperature Storage Solution- PBS with 0.02% NaN₃ • FEATURES: Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of antibodies. High affinity for kappa-light chain containing Ig antibodies from a variety of species. Protein L binds to all IgG subclasses from human, mouse and rat species. It also binds to human, mouse and rat IgM, IgA, IgE and IgD, as well as FAB with Kappa-light chains. Protein L is superior for binding chicken, hamster and pig IgG. |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Protein L Magnetic Beads are prepared by covalently coupling Recombinant Protein L (contains five Ig kappa light chain binding domains, BV catalog # 6530) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per ml of wet gel.
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Can the Protein L magnetic beads be re-used?
6537-1 Protein L magnetic beads could be re-used. However, we recommend re-using it with the same sample type to avoid possible cross contamination. We recommend using the below protocol for regeneration:
Regenerate the column by:
a. Washing with ~ 5 volumes of Elution Buffer.
b. Equilibrate with 5 volumes of Binding Buffer containing 0.2% sodium azide.
c. Store upright at 4 °C.
Regenerate the column by:
a. Washing with ~ 5 volumes of Elution Buffer.
b. Equilibrate with 5 volumes of Binding Buffer containing 0.2% sodium azide.
c. Store upright at 4 °C.
