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Product Details
| Cat # +Size | K546-10 |
|---|---|
| Size | 10 columns |
| Kit Summary | The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit. |
| Detection Method | Absorbance at 280 nm |
| Species Reactivity | Rat, Mouse, Human, Goat, Rabbit |
| Applications | Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
| Features & Benefits | • Ready-to-use pre-packed spin columns of 0.1 ml bead volume in 20% Ethanol. • HIGH BINDING CAPACITY: Binding of IgG ≥ 30 mg human, mouse or rabbit IgG mg/ml Protein G Agarose |
| Kit Components | • Elution Buffer Wash Buffer • Hi-Bind™ Protein G Spin-Column • Equilibration Buffer • Neutralization Buffer |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Protein G beads are widely used for IgG purification for their ability to bind selectively immunoglobulins. BioVision’s Protein G (Cat. No. 6510) is a genetically engineered protein containing three Ig-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The Hi-Bind™ Protein G-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. BioVision’s Protein G Spin Antibody Purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 3 mg of IgG per sample in ten samples (30 mg IgG total). The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
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BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
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