Protein G Sepharose

Reusable Protein G conjugated Sepharose beads with ≥20 mg/ml binding capacity & 2.07 ml/min flow rate for antibody purification from multiple sources
Catalog #: 6511 | abID: ab193259

Product Details

abID ab193259
Highlights • CONTENTS: Supplied as 50% slurry in 20 % Ethanol/H2O.
- HIGH BINDING CAPACITY: Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
- FLOW RATE TESTED*: 2.07 ml/min.
*Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
- USAGE: Reusable for up to 10 times without significant loss of binding capacity.
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein G-Sepharose beads are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose beads. BioVision Protein G (Cat. # 6510) is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G-Sepharose is >20 mg of human or rabbit IgG per ml of wet beads. Protein G-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
Can this Protein G Sepharose be used in centrifugation-based antibody purification?
No. It is based on gravity flow.
Adnan Shami Shah, PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling. Cell Rep., May 2019;  31091453.
Melina V. Jones, Effect of CXCR2 Inhibition on Behavioral Outcomes and Pathology in Rat Model of Neuromyelitis Optica. J Immunol Res, Dec 2018,   30648122.
Wicht et al., Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell. J. Virol., May 2014; 88: 4943 - 4952.
Jia et al., Solution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood Fluke Schistosoma mansoni.J. Biol. Chem.,  Mar 2014; 289: 7151 - 7163.
Price et al., CD8+ dendritic cell-mediated tolerance of autoreactive CD4+ T cells is deficient in NOD mice and can be corrected by blocking CD40L. J. Leukoc. Biol., Feb 2014; 95: 325 - 336.
For more citations of this product click here