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Protein G Sepharose Column

based on 1 citations in multiple journalsProtein G Sepharose Column14.1 4
Columns with Reusable Protein G conjugated Sepharose beads with ≥20 mg/ml binding capacity & 2.07 ml/min flow rate for antibody purification from multiple sources
Catalog #: 6518
SKU-Size Size Price Qty
6518-1 1 ml
6518-5 5 ml
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Product Details

Highlights • CONTENTS
- Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide.
- HIGH BINDING CAPACITY: Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
- FLOW RATE TESTED*: 2.07 ml/min.
*Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
- USAGE: Reusable for up to 10 times without significant loss of binding capacity.
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein G-Sepharose Columns are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose beads. BioVision Protein G (Cat. # 6510) is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G-Sepharose Column is >20 mg of human or rabbit IgG per ml of wet beads. Protein G-Sepharose Columns display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.

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Traini et al., Inhibition of Tumor Growth and Angiogenesis by SP-2, an Anti–Lectin, Galactoside-Binding Soluble 3 Binding Protein (LGALS3BP) Antibody. Mol. Cancer Ther., Apr 2014; 13: 916 - 925.
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