Protein Carbonyl Content Assay Kit

Simple assay to quantify protein carbonyls
Catalog #: K830 | abID: ab126287

Product Details

abID ab126287
Cat # +Size K830-100
Size 100 assays
Detection Method Absorbance ( 375 nm)
Species Reactivity Mammalian
Applications N/A
Features & Benefits • Simple procedure; takes ~ 30-90 minutes
Kit Components DNPH Solution
87% TCA Solution
10% Streptozocin Solution
6 M Guanidine Solution
96-Well Clear Plate
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375 nm. BioVision’s Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1 mg (~ 15 nmol) sample has a detection limit of about 0.15 nmol carbonyl, where BSA typically contains approximately 1-3 nmol carbonyl/mg.

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Do we need to use two columns for each labeling reaction? If so, do both columns need to go through the steps of VI-C-1 in the protocol? In other words, do we have to wash the resin for the two columns at the same time in one labeling reaction?
Yes, the end user needs two columns for each labeling reaction. This is to get rid of the free Cy5 completely and one column volume of bead is not enough. Yes, two columns must be washed with buffer as described in the datasheet.
Homogenization of tissue is suggested in your protocol using water is not working for us. What would you recommend to homogenize tissues instead?
We recommend using water only as mentioned in the protocol. Additionally, we recommend passing the cell lysate supernatant through 10 kDa spin column (10000 x g, 4 °C, 10 min; BioVision Cat#1997) and retain the filtrate for the assay.
Somanah, J. et al., (2017) Extracts of Mauritian Carica papaya (var solo) protect SW872 and HepG2 cells against hydrogen peroxide induced oxidative stress, Journal of Food Science and Technology, Apr.2017, 54(7), 1917-1927
Gaspard & McMaster. The Mitochondrial Quality Control Protein Yme1 Is Necessary to Prevent Defective Mitophagy in a Yeast Model of Barth Syndrome. J. Biol. Chem., Apr 2015; 290: 9284 - 9298.
Wilson et al., GCN2 is required to increase fibroblast growth factor 21 and maintain hepatic triglyceride homeostasis during asparaginase treatment.Am J Physiol Endocrinol Metab, Feb 2015; 308: E283 - E293.
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