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Protein A Spin Antibody Purification Kit

The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
Catalog #: K942

In stock

$325.00

Product Details

Cat # +Size K942-10
Size 10 columns
Kit Summary The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
Species Reactivity Human, mouse, rat, goat and rabbit
Applications Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
Features & Benefits • Ready-to-use pre-packed spin columns of 0.1 ml bead volume in 20% Ethanol.
• HIGH BINDING CAPACITY: Binding of IgG ≥ 30 mg human, mouse or rabbit IgG mg/ml Protein A-Agarose
Kit Components • Hi-Bind Protein A Spin-Column
• Equilibration Buffer
• Binding Buffer
• Elution Buffer
• Neutralization Buffer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins. BioVision’s Protein A (Cat. No. 6500) is a genetically engineered protein containing five IgG-binding regions of native Protein A. Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions. Hi-BindTM Protein A-Agarose beads (Cat. No. 6520) for purification of IgG contain covalently coupled recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein purification. BioVision’s Protein A Mini Antibody Purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 30 mg/ml of IgG. The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.


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