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Protein A Sepharose

based on 5 citations in multiple journalsProtein A Sepharose54.1 4
Reusable Protein A conjugated Sepharose beads with ≥16 mg/ml binding capacity & 2.07 ml/min flow rate for antibody purification from multiple sources
Catalog #: 6501
SKU-Size Size Price Qty
6501-1 1 ml
6501-5 5 ml
6501-25 25 ml
6501-100 100 ml
More Sizes Get Quote

Product Details

Highlights • CONTENTS
- Supplied as a 50% slurry in 20 % Ethanol/H2O.
- HIGH BINDING CAPACITY: Binding of IgG ≥16 mg human or rabbit IgG/ml Protein A-Sepharose.
- FLOW RATE TESTED*: 2.07 ml/min.
*Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
- USAGE: Reusable for up to 10 times without significant loss of binding capacity.
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein A-Sepharose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose beads. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Sepharose is ≥ 16 mg human or rabbit IgG per ml of wet beads. Protein A-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.

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I am using RIPA for my IP protocol. Is RIPA compatible with Protein A Sepharose beads?
RIPA or any buffer that contains detergents is not compatible with Sepharose beads. It is recommended to use buffers without detergents for your experiments.
Sury et al., Quantitative Proteomics Reveals Dynamic Interaction of c-Jun N-terminal Kinase (JNK) with RNA Transport Granule Proteins Splicing Factor Proline- and Glutamine-rich (Sfpq) and Non-POU Domain-containing Octamer-binding Protein (Nono) during Neuronal Differentiation. Mol. Cell. Proteomics, Jan 2015; 14: 50 - 65.
Preston et al., Implications of the E-selectin S128R mutation for drug discovery. Glycobiology, Jul 2014; 24: 592 - 601.
Schram, K. et. al. Regulation of MT1-MMP and MMP-2 by Leptin in Cardiac Fibroblasts Involves Rho/ROCK-Dependent Actin Cytoskeletal Reorganization and Leads to Enhanced Cell Migration. Endocrinology, May 2011; 152: 2037 - 2047.
Arana-Argáez, V., et al. Inhibitors of MAPK Pathway ERK1/2 or p38 Prevent the IL-1b-Induced Up-Regulation of SRP72 Autoantigen in Jurkat Cells. J. Biol. Chem., 2010; 285: 32824-32833.
Yang X et al (2009) J. Cell Sci.; 122: 2473 - 2480.
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