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Protein A Magnetic Beads

Reusable Protein A conjugated magnetic beads with ≥25 mg/ml binding capacity for antibody purification from multiple sources
Catalog #: 6507

Product Details

Cat # +Size 6507-1
Size 1 ml
Highlights • CONTENTS
- Supplied as a 50% slurry in 20% ethanol.
- Parameter Description:
Support Characteristics- Paramagnetic, spherical, 6 % cross-linked agarose
Ligand- Recombinant Protein A
Particle Size- 75 – 150 µm
Binding Capacity- Generally >25 mg human IgG/ml wet beads
Working Temperature-Room temperature
Storage Solution- 20% ethanol
Storage Temperature-4 – 8 °C
• FEATURES- Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of IgG antibodies. High affinity for Fc region of IgG antibodies from a variety of species. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A (contains five IgG binding domain, BV catalog # 6500B) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.

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Can the Protein A magnetic beads be re-used?
6507-1 Protein A magnetic beads could be re-used. However, we recommend re-using it with the same sample type to avoid possible cross contamination. We recommend using the below protocol for regeneration:
Regenerate the column by:
a. Washing with ~ 5 volumes of Elution Buffer.
b. Equilibrate with 5 volumes of Binding Buffer containing 0.2% sodium azide.
c. Store upright at 4 °C.