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Protein A (Lyophilized)

Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Catalog #: 6500B

In stock

SKU-Size Size Price Qty
6500B-10 10 mg
$90.00
6500B-25 25 mg
$185.00
6500B-100 100 mg
$595.00
6500B-1000 1 g
$1,595.00
More Sizes Get Quote

Product Details

Alternate Name Protein A
Gene ID 3919448
Accession # YP_498670
Source E. coli
Appearance Lyophilized
Physical Form Description Lyophilized powder
Molecular Weight 39.21 kDa
Purity by SDS-PAGE ≥97%
Purity by ≥97%
Endotoxin Level <0.1 ng/μg
Reconstitution Instructions Reconstitute in H₂O
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.


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Nagatsuka, Takehiro et al. (2017) Preparation of silicon nitride biochips for reflectometric interference spectroscopic (RIfS) analysis of biological toxins and E. coli O157:H7 strain, Sensors and Actuators B: Chemical, 2017 Jul;246:937-942
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