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Product Details
| Highlights | • CONTENTS: Supplied as a 50% slurry in 20% Ethanol/H₂O; >5 mg Protein A/G/L per ml of sepharose beads. • FEATURES: Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. The binding capacity is greater than 10 mg/ml of gel; High flow rate; Low falling off of rProtein A/G/L; pH stability 2-10. • APPLICATIONS: Purification of monoclonal and polyclonal antibodies, Immunoprecipitation. |
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| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Protein A/G/L-Sepharose is prepared by covalently coupling recombinant Protein A/G/L (Cat.# 6540; containing five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding regions of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding) to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet gel.
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